MAGUS CS5 Calibration Slide (XY0.01/0.07/0.15/0.6/1.5)

Calibration slide for microscopes.
The slide has four circles of 0.07mm, 0.15mm, 0.6mm, and 1.5mm in diameter etched on it. In the center of the slide, there are crosshairs – horizontal and vertical micrometer scales of 100 divisions. The scale division is 0.01mm and the length of each scale is 1mm.
The calibration slide (stage micrometer) is used to determine the magnification, linear field of view of the microscope, and the division value of the eyepiece scales and reticles.
With a digital camera, the slide is used to calibrate the image analysis software for each objective.

Calibrating the image analysis software

The calibration is performed for each objective that will be used in observations. The calibration slide is used to determine the calibration factor for the image analysis software.

Calibration procedure:

1. Fix the calibration slide on the microscope stage.
2. Select the desired objective and set the maximum camera resolution.
3. Get a contrast image of the scale on the monitor screen and capture the image.
4. Select “Calibration” mode in the image analysis software.
5. Click with the mouse to define the maximum visible distance between the first and last divisions of the scale.
6. Enter the resulting scale size in real units into the image analysis software.
7. Enter the calibration name by specifying the current objective magnification.
8. Check the result: In “Measurement” mode, measure a few divisions of the calibration slide scales in real units.
9. The image analysis software will save the calibration factor under the selected name. After that, it will automatically convert the results to the selected units of measurement.

Calibration of the eyepiece scale

The calibration of the eyepiece scale is performed for each objective that will be used in observations. The slide should be placed on the stage and the microscope should be focused on the scale. The eyepiece should be rotated in the eyepiece tube until the scales of a slide and an eyepiece are aligned parallel to each other.

If you use low-magnification objectives, you should calculate how many divisions of the eyepiece scale fit into the slide scale.
If you use medium and high-magnification objectives, you should calculate how many divisions of the slide fit into the scale of the eyepiece.

Calculation for each objective is carried out according to the formula:
Е=ТL/A, where
E – eyepiece division value
Т – stage division value specified on the stage micrometer (0.01mm)
L – number of stage micrometer divisions
А – number of eyepiece scale divisions.

The resulting values of the eyepiece scale division for each objective shall be recorded.

When measuring an object, first you should count the number of eyepiece scale divisions that the object covers. The result is multiplied by the scale value previously calculated for the selected objective. The resulting value will be the true linear value of the object.